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Human CD43 Alexa Fluor® 350-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2038U

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 290111

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD43
Ser20-Pro400
Accession # P16150

Specificity

Detects human CD43. Stains human CD43-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human CD43 Alexa Fluor® 350-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48 in mouse, is a 100-105 kDa type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells. Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. Mature CD43 shows 49% amino (aa) sequence identity between human and mouse.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Gene Symbol

SPN

UniProt

Additional CD43 Products

Product Documents for Human CD43 Alexa Fluor® 350-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD43 Alexa Fluor® 350-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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