PBMC lymphocytes were stained with Mouse Anti-Human CD46 Monoclonal Antibody (Catalog # MAB2005, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD46 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: see below

Western Blot

1 µg/mL
Sample: Recombinant Human CD46

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB2005 in the following applications:

Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD46

CD46, also known as MCP, is a type I membrane protein that functions as a cofactor for Complement Factor I-mediated inactivation of complement components C3b and C4b. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. The extracellular region of CD46 contains four tandem Sushi domains. At least fourteen transcript variants encode isoforms with deletions or substitutions following the fourth Sushi domain. Within corresponding regions, human CD46 shares 50% amino acid sequence identity with mouse and rat CD46.

Alternate Names

CD46, MCP

Entrez Gene IDs

4179 (Human); 17221 (Mouse)

Gene Symbol

CD46

UniProt

NP_722548

Additional CD46 Products

Product Documents for Human CD46 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD46 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry