Human CD5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1636
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Simple Western, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD5
Arg25-Asn371
Accession # P06127
Arg25-Asn371
Accession # P06127
Specificity
Detects human CD5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CD5 Antibody
Detection of Human CD5 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD5 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD5 in Human PBMCs.
CD5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human CD5 by Simple WesternTM.
Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for CD5 at approximately 93 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human CD5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood lymphocytes
Sample: Human whole blood lymphocytes
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells
Sample: Immersion fixed human peripheral blood mononuclear cells
Simple Western
10 µg/mL
Sample: CEM human T-lymphoblastoid cell line
Sample: CEM human T-lymphoblastoid cell line
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD5
CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.
References
- Ledbetter, J.A. et al. 1985, J. Immunol. 135:2331.
- Damle, N.K. et al. 1988, J. Immunol. 140:1753.
Alternate Names
CD5
Gene Symbol
CD5
UniProt
Additional CD5 Products
Product Documents for Human CD5 Antibody
Product Specific Notices for Human CD5 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...