Human Ramos Burkitt's lymphoma cell line was stained with Mouse Anti-Human CD72 Monoclonal Antibody (Catalog # MAB5405, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CD72 in Ramos Human Cell Line.

CD72 was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human CD72 Monoclonal Antibody (Catalog # MAB5405) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CD72 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Ramos Human Burkitt's Lymphoma Cell Line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD72

CD72, also known as Lyb‑2, is a 40‑45 kDa type II transmembrane glycoprotein that plays a role in immune system regulation (1). Mature human CD72 consists of a 95 amino acid (aa) cytoplasmic domain with two immunoreceptor tyrosine‑based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain with a coiled‑coil domain and a C‑type lectin domain (2). Within the ECD, human CD72 shares 48% and 44% aa sequence identity with mouse and rat CD72, respectively. CD72 is expressed on B lineage cells, NK cells, monocytes, dendritic cells, and mast cells (2‑6). CD72 binds to CD5 with mouse/human cross‑reactivity and to Semaphorin 4D/CD100 (5, 7‑9). It associates with CD79A in the B cell antigen receptor (BCR) complex following antigen stimulation and dampens BCR signaling through interactions with the phosphatase SHP‑1 (10). CD72 ligation with antibodies or with Semaphorin 4D induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP‑1, leading to B cell proliferation (5, 9). Both CD72 and Semaphorin 4D are required for the maintenance of B cell anergy and the regulation of peripheral B cell tolerance as shown by the development of autoimmunity in mice that lack either molecule (10, 11). In addition to its negative regulation of BCR signaling, CD72 can induce positive signaling in B cells independent of the BCR (12). CD72 binding to Semaphorin 4D induces cytokine production by monocytes and dendritic cells, inhibits SCF R/c‑kit induced mast cell proliferation and activation, and inhibits the cytolytic activity of NK cells (4‑6). Semaphorin 4D is expressed on activated NK cells and contributes to the adhesive interaction between NK and CD72⁺ target cells leading to a more efficent killing and enhanced IFN-gamma secretion (13).

References

Nykimbeng-Takwi, E. and S.P. Chapoval (2011) Immunol. Res. 50:10.
Von Hoegen, I. et al. (1990) J. Immunol. 144:4870.
Nakayama, E. et al. (1989) Proc. Natl. Acad. Sci. 86:1352.
Alcon, V.L. et al. (2009) Eur. J. Immunol. 39:826.
Ishida, I. et al. (2003) Int. Immunol. 15:1027.
Kataoka, T.R. et al. (2010) J. Immunol. 184:2468.
Van de Velde, H. et al. (1991) Nature 351:662.
Luo, W. et al. (1992) J. Immunol. 148:1630.
Kumanogoh, A. et al. (2000) Immunity 13:621.
Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
Li, D. H.-H. et al. (2008) Arthritis Rheum. 58:3192.
Wu, H.-J. et al. (2001) J. Immunol. 167:1263.
Mizrahi, S. et al. (2007) PLoS ONE 9:e818.

Alternate Names

CD72, Ly-19.2, Ly-32.2, Lyb-2

Entrez Gene IDs

971 (Human); 12517 (Mouse)

Gene Symbol

CD72

UniProt

P21854

Additional CD72 Products

Product Documents for Human CD72 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD72 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD72 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5405

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD72 Antibody

Detection of CD72 in Human Ramos Cell Line by Flow Cytometry.