Skip to main content

Human CD74 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB35901R

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 332516

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-S-derived recombinant human CD74
Gln73-Met232
Accession # NP_004346

Specificity

Detects recombinant human CD74 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD74 Alexa Fluor® 647-conjugated Antibody

Detection of CD74 antibody in Human PBMC antibody by Flow Cytometry.

Detection of CD74 in Human PBMC by Flow Cytometry.

Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CD74 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB35901R) or (B) Mouse IgG1 isotype control (IC002R) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Detection of CD74 antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of CD74 in K562 Human Cell Line by Flow Cytometry.

K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human CD74 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB35901R, filled histogram) or Mouse IgG1 isotype control (IC002R,open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human CD74 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human PBMC lymphocytes and K562 myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD74

CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappaB. CD74 is up-regulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type 1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Gene Symbol

CD74

UniProt

Additional CD74 Products

Product Documents for Human CD74 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD74 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...