Human peripheral blood platelets were stained with Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB1880, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of Human CD9 by Western Blot

Validation of protein expression by Western blotting.All four exosome samples and their corresponding cell lines were used for validation. Furthermore, supernatant from the pelleted exosomes was used as a control. The selected proteins FASN, XPO1, CD9 and PDCD6IP, were tested with ENO1 and GAPDH as controls. PSA was tested to confirm it is secreted through alternative secretion pathway and therefore not present within exosomes. The nearest protein marker (kDa) is indicated for each blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24391718), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD9 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human platelets

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD9

CD9, also known as motility-related protein-1 (MRP-1), is a tetraspan family glycoprotein expressed by a variety of hematopoietic and epithelial cells. It forms homotypic associations as well as heterotypic associations with other tetraspan proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Entrez Gene IDs

928 (Human); 12527 (Mouse)

Gene Symbol

CD9

UniProt

P21926

Additional CD9 Products

Product Documents for Human CD9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD9 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1880

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD9 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD9 Antibody

Detection of CD9 in Human Platelets by Flow Cytometry.