Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD94 Monoclonal Antibody (Catalog # MAB10581) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by anti-Mouse IgG Allophycocyanin-conjugated secondary antibody (F0101B) and Mouse Anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD94 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human Blood Lymphocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8⁺ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References

Chester, C. et al. (2015) Front. Immunol. 6:601.
Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
Lieto, L.D. et al. (2006) Genes Immun. 7:36.
Yu, J. et al. (2010) Blood 115:274.
Phillips, J.H. et al. (1996) Immunity 5:163.
Lazetic, S. et al. (1996) J. Immunol. 157:4741.
Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
Braud, V.M. et al. (1998) Nature 391:795.
Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
Houchins, J.P. et al. (1997) J. Immunol. 158:3603.

Alternate Names

CD94, KLRD1

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Gene Symbol

KLRD1

UniProt

Q13241.2

Additional CD94 Products

Product Documents for Human CD94 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD94 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD94 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10581

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD94 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD94 Antibody

Detection of CD94 in Human Blood Lymphocytes by Flow Cytometry.