Human CD96 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6199
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD96
Lys25-Asp501
Accession # NP_005807
Lys25-Asp501
Accession # NP_005807
Specificity
Detects human CD96 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse CD96 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD96 Antibody
Detection of Human CD96 by Western Blot.
Western blot shows lysates of human Th2 cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human CD96 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human Th2 cells
Sample: Human Th2 cells
Reviewed Applications
Read 1 review rated 4 using AF6199 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD96
CD96 (also Tactile) is a 160 kDa member of the Ig-Superfamily. It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells. Human CD96 binds to CD155 and presumably participates in NK cell killing of CD155-expressing target cells. Mature human CD96 is a 564 amino acid (aa), type I transmembrane glycoprotein. It contains a 498 aa extracellular region (aa 22-519) that contains three Ig-like domains. The two N-terminal domains are V-type (aa 38-238), while the distal domain is a C-type structure (aa 269-375). There is one isoform that shows a deletion of aa 183-192. This deletion converts the second V-type domain into an I‑like domain, and generates the most common form of CD96. An additional isoform shows the same deletion coupled to a nine aa substitution for aa 410-585. Over aa 1-536, human CD96 shares 59% aa identity with mouse CD96.
Alternate Names
CD96, TACTILE
Gene Symbol
CD96
UniProt
Additional CD96 Products
Product Documents for Human CD96 Antibody
Product Specific Notices for Human CD96 Antibody
For research use only
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