Human CDCP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2666
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 309137
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Ala33-Leu666
Accession # NP_073753
Ala33-Leu666
Accession # NP_073753
Specificity
Detects human CDCP1 in direct ELISAs and Western blots. Recognizes an epitope found between aa 33‑369 of human CDCP1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human CDCP1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CDCP1, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human CDCP1, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human CDCP1
Sample: Recombinant Human CDCP1
Reviewed Applications
Read 1 review rated 4 using MAB2666 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDCP1
CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.
Long Name
CUB Domain Containing Protein 1
Alternate Names
CD318, SIMA135
Gene Symbol
CDCP1
UniProt
Additional CDCP1 Products
Product Documents for Human CDCP1 Antibody
Product Specific Notices for Human CDCP1 Antibody
For research use only
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