Human CEACAM-16 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9885

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB9885-100
MAB9885-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2747B

View all CEACAM-16 Antibodies »

Product Summary for Human CEACAM-16 Antibody

Immunogen

Chinese Hamster Ovary cell line CHO-derived human CEACAM-16
Met1-Gly425
Accession # Q2WEN9

Specificity

Detects human CEACAM-16 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CEACAM-16 Antibody

Detection of CEACAM-16 in HEK293 Human Cell Line Transfected with Human CEACAM-16 by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with human CEACAM-16 (filled histogram) or irrelevant protein (open histogram) was stained with Rabbit Anti-Human CEACAM-16 Monoclonal Antibody (Catalog # MAB9885) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

CEACAM-16 in HEK293 Human Cell Line.

CEACAM-16 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rabbit Anti-Human CEACAM-16 Monoclonal Antibody (Catalog # MAB9885) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CEACAM-16 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human CEACAM-16

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-16

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16 (CEACAM-16), or CEAL2, is part of the CEA protein family consisting of CEACAMs and the pregnancy‑specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Unique to the CEA family, CEACAM-16 is a secreted molecule lacking a recognizable transmembrane domain or GPI anchor (3). Mature human CEACAM-16 is 405 amino acids (aa), containing 2 IgC2-like domains and 2 IgV-like domains. CEACAM-16 is one of only five conserved CEACAMs among mice, rats, and humans (2). Mature human CEACAM-16 shares 90% and 89% aa identity with mouse and rat CEACAM‑16, respectively. Originally discovered as a biomarker for colorectal cancer (4), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5-7). CEACAM-16 is specifically expressed in the inner ear and has been shown to play a critical role in hearing. CEACAM-16 has been identified as a binding partner for alpha tectorin and specific mutations in CEACAM-16 have been linked to autosomal dominant nonsyndromic deafness (ADNSHL) (3, 8).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R. et al. (2005) Genomics 86:566.
  3. Zheng, J. et al. (2011) PNAS. 108(10):4218.
  4. Gold P. and Freedman S.O. (1965) J Exp Med 122:467.
  5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  6. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  7. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
  8. Wang, H. et al. (2015) J Hum Genet. 60(3):119.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16

Alternate Names

CEAL2, DFNA4B

Entrez Gene IDs

388551 (Human); 330483 (Mouse); 292700 (Rat)

Gene Symbol

CEACAM16

UniProt

Q2WEN9

Additional CEACAM-16 Products

Product Documents for Human CEACAM-16 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEACAM-16 Antibody

For research use only

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