Human CEBP alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7094
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CEBP alpha
Met1-Ala124
Accession # P49715
Met1-Ala124
Accession # P49715
Specificity
Detects human CEBP alpha in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CEBP beta, rhCEBP gamma, rhCEBP delta, rhCEBP zeta, and rhCEBP epsilon is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CEBP alpha Antibody
CEBP alpha in Human Liver.
CEBP a was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CEBP a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7094) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence
Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha+marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha+, CD146−) and in the endothelial-like cells (CEBP alpha−, CD146+). (c) CD51+, NES+, PDGFR alpha+ mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence
Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha+marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha+, CD146−) and in the endothelial-like cells (CEBP alpha−, CD146+). (c) CD51+, NES+, PDGFR alpha+ mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human CEBP alpha Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEBP alpha
CEBP alpha (CCAAT/Enhancer-Binding Protein; also C/EBP) is a 42-43 kDa member of the C/EBP subfamily, bZIP family of proteins. It is expressed in multiple cell types, particularly those that are fully differentiated, and includes hepatocytes, skeletal muscle cells, adipocytes, intestinal epithelium and proliferating macrophages. CEBP alpha acts as a transcriptional activator for a large and diverse number of genes, including glycogen synthase, GLUT4, and G-CSFR, and participates in both cell cycle progression and differentiation. Human CEBP alpha is 358 amino acids (aa) in length). It contains one transactivation domain (aa 1-96), poly-Gly segment (aa 99-104), a poly-Pro motif (aa 183-189), a bZIP dimerization region (aa 281-334), and an overlapping DNA-binding Leu-zipper domain (aa 317-345). CEBP alpha forms both homodimers, and heterodimers with other subfamily members. One alternative start site at Met120 generates a low activity 30-32 kDa isoform variant. There is also a utilized phosphorylation site at Ser21 that regulates activity. Over aa 1-124, human CEBP alpha shares 93% aa identity with mouse CEBP alpha.
Long Name
CCAAT/enhancer-Binding Protein alpha
Alternate Names
C/EBP-alpha, CEBPA
Gene Symbol
CEBPA
UniProt
Additional CEBP alpha Products
Product Documents for Human CEBP alpha Antibody
Product Specific Notices for Human CEBP alpha Antibody
For research use only
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