Human Choline Acetyltransferase/ChAT Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3447
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Choline Acetyltransferase/ChAT isoform R
Ala2-Pro630
Accession # NP_066266
Ala2-Pro630
Accession # NP_066266
Specificity
Detects human Choline Acetyltransferase/ChAT in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Choline Acetyltransferase/ChAT Antibody
Choline Acetyltransferase/ChAT in Human Brain.
Choline Acetyltransferase/ChAT was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human Choline Acetyltransferase/ChAT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3447) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Choline Acetyltransferase/ChAT Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Western Blot
0.1 µg/mL
Sample: Recombinant Human Choline Acetyltransferase/ChAT Isoform R
Sample: Recombinant Human Choline Acetyltransferase/ChAT Isoform R
Reviewed Applications
Read 2 reviews rated 4.5 using AF3447 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Choline Acetyltransferase/ChAT
Alternate Names
CHAT, Choline acetylase
Gene Symbol
CHAT
UniProt
Additional Choline Acetyltransferase/ChAT Products
Product Documents for Human Choline Acetyltransferase/ChAT Antibody
Product Specific Notices for Human Choline Acetyltransferase/ChAT Antibody
For research use only
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