Human Chymotrypsin C/CTRC Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6907
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Chymotrypsin C/CTRC
Cys17-Leu268
Accession # Q99895
Cys17-Leu268
Accession # Q99895
Specificity
Detects human Chymotrypsin C/CTRC in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Chymotrypsin C/CTRC Antibody
Detection of Human Chymotrypsin C/CTRC by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chymotrypsin C/CTRC at approximately 33-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chymotrypsin C/CTRC in Human Pancreas.
Chymotrypsin C/CTRC was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Chymotrypsin C/CTRC Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Western Blot
1 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chymotrypsin C/CTRC
References
- Tomomura, A. et al. (1995) J. Biol. Chem. 270:30315.
- Szmola, R. and M. Sahin-Toth (2007) Proc. Natl. Acad. Sci. USA 104:11227.
- Nemoda, Z. and M. Sahin-Toth (2006) J. Biol. Chem. 281:11879.
- Hasegawa, H. et al. (2010) J. Biol. Chem. 285:25448.
Alternate Names
Caldecrin, CLCR, CTRC, ELA4, Elastase IV
Gene Symbol
CTRC
UniProt
Additional Chymotrypsin C/CTRC Products
Product Documents for Human Chymotrypsin C/CTRC Antibody
Product Specific Notices for Human Chymotrypsin C/CTRC Antibody
For research use only
Loading...
Loading...
Loading...
Loading...