Human CIB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7557
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 791119
Product Specifications
Immunogen
E. coli-derived recombinant human CIB1
Gly2-Leu191
Accession # E9Q3T6
Gly2-Leu191
Accession # E9Q3T6
Specificity
Detects human CIB1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CIB1 Antibody
Detection of Human CIB1 by Western Blot.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CIB1 in Human Breast Cancer Tissue.
CIB1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CIB1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
2 µg/mL
Sample: Human kidney tissue
Sample: Human kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CIB1
Long Name
Calcium and Integrin Binding 1
Alternate Names
Calmyrin, CIBP, KIP, PRKDCIP, SIP2-28
Gene Symbol
CIB1
UniProt
Additional CIB1 Products
Product Documents for Human CIB1 Antibody
Product Specific Notices for Human CIB1 Antibody
For research use only
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