Human CILP-1 C-Terminal Fragment Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4477
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CILP‑1 C-Terminal Fragment
Arg724-Asn1184
Accession # O75339
Arg724-Asn1184
Accession # O75339
Specificity
Detects human CILP‑1 C-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 N-terminal peptide is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human CILP-1 C-Terminal Fragment Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CILP-1 C-Terminal Fragment
Sample: Recombinant Human CILP-1 C-Terminal Fragment
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CILP-1
The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725‑1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively.
Long Name
Cartilage Intermediate Layer Protein 1
Alternate Names
CILP, CILP1
Gene Symbol
CILP
UniProt
Additional CILP-1 Products
Product Documents for Human CILP-1 C-Terminal Fragment Antibody
Product Specific Notices for Human CILP-1 C-Terminal Fragment Antibody
For research use only
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