HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Claudin‑6 in iPS2 Human Induced Pluripotent Stem Cells.

Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Claudin-6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cells differentiated into definitive endoderm using StemXVivo Endoderm Kit (Catalog # SC019)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-6

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Alternate Names

Claudin6, CLDN6, Skullin 2

Entrez Gene IDs

9074 (Human); 54419 (Mouse); 287098 (Rat)

Gene Symbol

CLDN6

UniProt

P56747

Additional Claudin-6 Products

Product Documents for Human Claudin-6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Claudin-6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Claudin-6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3656

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Claudin-6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Claudin-6 Antibody

Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry.