Human Coagulation Factor V Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB98562
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 986237
Product Summary for Human Coagulation Factor V Antibody
Immunogen
Human Coagulation Factor V synthetic peptide
Specificity
Detects human Coagulation Factor V in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Coagulation Factor V Antibody
Human Coagulation Factor V ELISA Standard Curve.
Recombinant Human Coagulation Factor V protein was serially diluted 2-fold and captured by Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Coagulation Factor V Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor V
Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.
Long Name
Coagulation Factor V
Alternate Names
Cf-5, Cf5, Coagulation Factor 5, F5, Factor V, FVL, PCCF, Proaccelerin, Labile Factor, RPRGL1, THPH2
Gene Symbol
F5
Additional Coagulation Factor V Products
Product Documents for Human Coagulation Factor V Antibody
Product Specific Notices for Human Coagulation Factor V Antibody
For research use only
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