Human Coagulation Factor X Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1063
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rat
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 156106
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor X
Leu24-Lys488 (predicted)
Accession # P00742
Leu24-Lys488 (predicted)
Accession # P00742
Specificity
Detects human Coagulation Factor X in direct ELISAs and Western blots. It recognizes both Factor X and Factor Xa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Coagulation Factor X Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor X (Catalog # 1063-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor X (Catalog # 1063-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample:
Sample:
Recombinant Human Coagulation Factor X (Catalog # 1063-SE)
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB1063 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor X
As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X (rhFX) is initially synthesized in the liver as a single-chain precursor of 488 amino acids (aa) with a signal peptide and a pro region (aa 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (aa 41‑179) and heavy (aa 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain.
Alternate Names
Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor
Gene Symbol
F10
UniProt
Additional Coagulation Factor X Products
Product Documents for Human Coagulation Factor X Antibody
Product Specific Notices for Human Coagulation Factor X Antibody
For research use only
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