Human Collagen XIII  alpha1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6346

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB6346
MAB6346-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 584718

View all Collagen XIII alpha 1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Collagen XIII alpha1
Glu109-Pro668
Accession # Q5TAT6

Specificity

Detects human Collagen XIII alpha1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Collagen XIII alpha1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Collagen XIII  alpha1 Antibody

Collagen XIII a1 antibody in Human Placenta by Immunohistochemistry (IHC-P).

Collagen XIII alpha1 in Human Placenta.

Collagen XIII a1 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Collagen XIII a1 Monoclonal Antibody (Catalog # MAB6346) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Collagen XIII  alpha1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XIII alpha 1

COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the Integrin alpha1 subunit, Nidogen-2, and Fibronectin. Human COL13A1 is 717 amino acids (aa) in length. It has an N‑terminal 44 aa cytoplasmic region plus a 656 aa extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, aa 217-269, aa 442-463, and aa 700-717) interspersed among three collagenous (COL) domains (aa 122-216, aa 270-441, and aa 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, aa 551-565, and aa 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1.

Alternate Names

COL13A1

Entrez Gene IDs

1305 (Human); 12817 (Mouse); 499431 (Rat)

Gene Symbol

COL13A1

UniProt

Q5TAT6 (Human)

Additional Collagen XIII alpha 1 Products

Product Documents for Human Collagen XIII  alpha1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Collagen XIII  alpha1 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.