Skip to main content

Human Complement Component C3aR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5995

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB5995
MAB5995-SP

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 534625

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human C3a R
Met1-Val482
Accession # Q16581

Specificity

Detects human Complement Component C3a R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Complement Component C3aR Antibody

Detection of Complement Component C3a R antibody in Human Monocytes antibody by Flow Cytometry.

Detection of Complement Component C3a R in Human Monocytes by Flow Cytometry.

Human blood-derived monocytes were stained with Mouse Anti-Human Complement Component C3a R Monoclonal Antibody (Catalog # MAB5995, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human Complement Component C3aR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human blood-derived monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C3aR

Complement Component 3a receptor (C3a R) is an ~90 kDa G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Long Name

Complement Component C3a Receptor

Alternate Names

AZ3B, C3AR, C3AR1, C3R1, Complement Component C3a R

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Gene Symbol

C3AR1

UniProt

Additional Complement Component C3aR Products

Product Documents for Human Complement Component C3aR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Complement Component C3aR Antibody

For research use only

Loading...
Loading...
Loading...
Loading...
Loading...