Human Complement Component C9 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8126
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived human Complement Component C9
Gln22-Lys559 (predicted)
Accession # P02748
Gln22-Lys559 (predicted)
Accession # P02748
Specificity
Detects human Complement Component C9 in directs ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Complement Component C9 Antibody
Detection of Human Complement Component C9 by Western Blot.
Western blot shows lysates of human plasma. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Complement Component C9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Complement Component C9 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: Human plasma
Sample: Human plasma
Reviewed Applications
Read 1 review rated 5 using AF8126 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C9
Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.
Alternate Names
complement component 9, complement component C9
Gene Symbol
C9
UniProt
Additional Complement Component C9 Products
Product Documents for Human Complement Component C9 Antibody
Product Specific Notices for Human Complement Component C9 Antibody
For research use only
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