Human Complement Factor H-related 5/
CFHR5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3845
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
ELISA, Immunocytochemistry, Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 390513
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor H-related 5/CFHR5
Glu19-Glu569
Accession # Q9BXR6
Glu19-Glu569
Accession # Q9BXR6
Specificity
Detects human Complement Factor H-related 5/CFHR5 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Complement Factor H-related 5/CFHR5 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor H‑related 5/CFHR5 (Catalog # 3845-F5), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor H‑related 5/CFHR5 (Catalog # 3845-F5), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Complement Factor H-related 5/CFHR5 (Catalog # 3845-F5) under non-reducing conditions only
Sample: Recombinant Human Complement Factor H-related 5/CFHR5 (Catalog # 3845-F5) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H-related 5/CFHR5
Complement factor H-related protein 5 (FHR-5 or CFHR5) is a 65 kDa member of the factor H-related protein family. It is secreted by the liver and circulates either free or bound to HDL. It is unique among FHR proteins in that it associates with complexes that arise from complement activation. It promotes C3b cleavage and blocks C3 convertase activity. Human CFHR5 is 551 amino acids (aa) in length. It contains nine, 60 aa SUSHI-, or short consensus repeats, plus 18 intrachain disulfide bonds. SUSHI repeats 5-7 (aa 267-444) reportedly mediate CFHR5 binding to CRP and heparin. The complement interaction site(s) is unknown. Human CFHR5 shares 64% aa sequence identity with canine CFHR5.
Alternate Names
CFHL5, CFHR5, Complement Factor Hrelated 5, FHR5
Entrez Gene IDs
81494 (Human)
Gene Symbol
CFHR5
UniProt
Additional Complement Factor H-related 5/CFHR5 Products
Product Documents for Human Complement Factor H-related 5/CFHR5 Antibody
Product Specific Notices for Human Complement Factor H-related 5/CFHR5 Antibody
For research use only
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