Human Connexin 43/GJA1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7737A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 578618

View all Connexin 43/GJA1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0 transfected with human Connexin 43/GJA1
Accession # P17302

Specificity

Detects human Connexin 43/GJA1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Connexin 43/GJA1 APC-conjugated Antibody

Detection of Connexin 43/GJA1 antibody in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP antibody by Flow Cytometry.

Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP was stained with and either (A) Mouse Anti-Human Connexin 43/GJA1 APC-conjugated Monoclonal Antibody (Catalog # FAB7737A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Connexin 43/GJA1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using FAB7737A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Connexin 43/GJA1

Connexin43 (Cx43; also gap junction alpha-1 protein) is a 41 - 44 kDa member of the connexin family, alpha-type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

References

  1. Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
  2. Willecke, K. et al. (2002) Biol. Chem. 383:725.
  3. Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
  4. Vinken, M. et al. (2006) Cell. Signal. 18:592.
  5. Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
  6. Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
  7. Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Entrez Gene IDs

2697 (Human)

Gene Symbol

GJA1

UniProt

P17302

Additional Connexin 43/GJA1 Products

Product Documents for Human Connexin 43/GJA1 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Connexin 43/GJA1 APC-conjugated Antibody

For research use only

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