Human Corin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2209
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 231443
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Corin
Ser67-Asn1042
Accession # Q9Y5Q5
Ser67-Asn1042
Accession # Q9Y5Q5
Specificity
Detects human Corin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Corin Antibody
Corin in HK2 Human Cell Line.
Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human Corin ELISA Standard Curve.
Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Corin Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Corin DuoSet ELISA Kit (Catalog # DY2209) for convenient development of a sandwich ELISA or the Human Corin Quantikine ELISA Kit (Catalog # DCRN00) for a complete optimized ELISA.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HK2 human kidney proximal tubule cell line
Sample: Immersion fixed HK2 human kidney proximal tubule cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB2209 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Corin
Alternate Names
ATC2, Pro-ANP-Converting Enzyme
Entrez Gene IDs
10699 (Human)
Gene Symbol
CORIN
UniProt
Additional Corin Products
Product Documents for Human Corin Antibody
Product Specific Notices for Human Corin Antibody
For research use only
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