Human CRLR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10044
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 998820
Product Specifications
Immunogen
Synthetic peptide containing human CALCRL
Accession # Q16602
Accession # Q16602
Specificity
Detects human CALCRL in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human CRLR Antibody
CRLR in KG-1 Human Cell Line.
CRLR was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CRLR in Human Lung.
CRLR was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CRLR Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed KG-1 human acute myelogenous leukemia cell line
Sample: Immersion fixed KG-1 human acute myelogenous leukemia cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung
Sample: Immersion fixed paraffin-embedded sections of human lung
Reviewed Applications
Read 1 review rated 5 using MAB10044 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRLR
Long Name
Calcitonin Receptor-like Receptor
Alternate Names
CALCRL, CGRPR
Gene Symbol
CALCRL
UniProt
Additional CRLR Products
Product Documents for Human CRLR Antibody
Product Specific Notices for Human CRLR Antibody
For research use only
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