Human CSL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4079
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CSL
Gly208-Ser500
Accession # Q06330
Gly208-Ser500
Accession # Q06330
Specificity
Detects human CSL in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CSL Antibody
CSL in U937 Human Cell Line.
CSL was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human CSL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4079) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CSL Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human CSL
Sample: Recombinant Human CSL
Reviewed Applications
Read 1 review rated 5 using AF4079 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CSL
CSL (CBF1/RBP-Jk; Su(H)/Suppressor of Hairless; Lag-1) is a 56 kDa member of the Su(H) family of transcription factors. It is constitutively bound to DNA where it participates in gene repression. Upon ligand binding, the intracellular domain of Notch translocates to the nucleus and interacts with CSL, allowing transcription of Notch target genes. Human CSL is 500 amino acids (aa) in length. It contains three DNA binding sites (aa 58‑65; 192‑201; and 265‑297) and one IPT domain (aa 355‑445). Multiple splice variants exist that involve the first 89 N-terminal amino acids. One variant (isoform 2) is 487 aa in length and shows a 6 aa substitution for amino acids 1‑19. Over amino acids 195‑487, human CSL isoform 2 shares more than 98% aa sequence identity with mouse, rat, and canine CSL.
Long Name
Recombining Binding Protein Supressor of Hairless [CBF1, CSL]
Alternate Names
CBF1, IGKJRB1, KBF2, RBP-JK, RBPJ, RBPSUH
Gene Symbol
RBPJ
UniProt
Additional CSL Products
Product Documents for Human CSL Antibody
Product Specific Notices for Human CSL Antibody
For research use only
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