Human CtBP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4639
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CtBP1
Met1-Leu440
Accession # Q13363
Met1-Leu440
Accession # Q13363
Specificity
Detects human and mouse CtBP1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CtBP2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CtBP1 Antibody
Detection of Human CtBP1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human CtBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CtBP1 and CtBP2 (5 ng/lane) were included. A specific band for CtBP1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CtBP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and PT18 mouse mast/basophil cell line
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and PT18 mouse mast/basophil cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CtBP1
CtBP1 (C terminal binding protein 1) was originally identified through its binding to the Adenovirus E1A oncoprotein via a 5 amino acid motif, PLDLS, which repressed E1A induced oncogenesis and cellular transformation. In the nucleus, CtBP1 functions predominantly as a transcriptional corepressor in conjunction with a variety of DNA binding repressors.Interaction with Pnn/DRS may modulate repression of E-cadherin by CtBP1. Recruitment of CtBP1 and HDAC to Smad1/Smad4 complexes by Smad6 results in inhibition of BMP stimulated gene expression. The interaction of CtBP1 with polycomb group proteins suggests it is a component of the chromatin remodeling machinery and transcriptional repression. Cytosolic CtBP1 has also been shown to be involved with CNS synapses, membrane trafficking, and regulation of the microtubule cytoskeleton.
Long Name
C-terminal-Binding Protein 1
Alternate Names
BARS
Gene Symbol
CTBP1
UniProt
Additional CtBP1 Products
Product Documents for Human CtBP1 Antibody
Product Specific Notices for Human CtBP1 Antibody
For research use only
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