Human CTGF/CCN2 C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB660
C-terminal Peptide
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Bioassay, ELISA Development, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 88430
Product Specifications
Immunogen
E. coli-derived recombinant human CTGF
Glu247-Ala349
Accession # NP_001892.1
Glu247-Ala349
Accession # NP_001892.1
Specificity
Detects human CTGF C-teriminal peptide in direct ELISAs. Does not cross-react with recombinant human (rh) LDGF, rhNOV, rhPDGF, rhPlGF, rhVEGF206, rhVEGF-B186, rhVEGF-C, rhVEGF-D, or rhWISP-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CTGF/CCN2 C-Terminus Antibody
CTGF/CCN2 in Human Breast Cancer Tissue.
CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human CTGF/CCN2 C-Terminus Monoclonal Antibody (Catalog # MAB660) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human CTGF/CCN2 by Immunohistochemistry
Immunostaining of neuroendocrine tumors. Representative photomicrographs demonstrating anti-connective tissue growth factor (CCN2) (panel A) immunostaining of gut carcinoid tumor tissue. CCN2 immunoreactivity was seen in cells corresponding to chromogranin A-positive tumor cells and gut smooth muscle (denoted by arrows in panels B and C, respectively). All sections were counterstained with hematoxylin. Magnification ×50. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20053285), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human CTGF/CCN2 C-Terminus Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using MAB660 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CTGF/CCN2
Long Name
Connective Tissue Growth Factor
Alternate Names
CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2
Gene Symbol
CCN2
UniProt
Additional CTGF/CCN2 Products
Product Documents for Human CTGF/CCN2 C-Terminus Antibody
Product Specific Notices for Human CTGF/CCN2 C-Terminus Antibody
For research use only
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