Human CXCL7/NAP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF393
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Dot Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL7/NAP-2
Ala59-Asp128
Accession # P02775
Ala59-Asp128
Accession # P02775
Specificity
Detects human CXCL7/NAP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 10% cross-reactivity with recombinant human (rh) GRO alpha, rhIL-8, rhCINC-2 alpha, and rhGRO beta is observed, and 5% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta, rhGCP-2, rmKC, rhMIG, and rhGRO beta. is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CXCL7/NAP-2 Antibody
Detection of Human CXCL7/NAP‑2 by Simple WesternTM.
Simple Western lane view shows lysates of human blood platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP-2 at approximately 7 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL7/NAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human CXCL7/NAP-2 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human blood platelets
Sample: Human blood platelets
Western Blot
0.1 µg/mL
Sample: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)
Sample: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/NAP-2
References
- Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
- Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.
Alternate Names
CTAP III, NAP-2, NAP2, PPBP
Entrez Gene IDs
5473 (Human)
Gene Symbol
PPBP
UniProt
Additional CXCL7/NAP-2 Products
Product Documents for Human CXCL7/NAP-2 Antibody
Product Specific Notices for Human CXCL7/NAP-2 Antibody
For research use only
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