Human Cyclin B2 Antibody

Name: Human Cyclin B2 Antibody
Brand: R&D Systems
SKU: AF6204

R&D Systems, part of Bio-Techne | Catalog # AF6204

Submit a review

Citations (1)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Cyclin B2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclin B2
Met1-Lys101
Accession # O95067

Specificity

Detects human Cyclin B2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Cyclin B2 Antibody

Detection of Human Cyclin B2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cyclin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6204) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyclin B2 at approximately 48-54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cyclin B2 in Human Colon.

Cyclin B2 was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human Cyclin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6204) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in mucosal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cyclin B2 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 48-54 kDa member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201-290 and 298-383) and two substrate binding sites (aa 165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Alternate Names

CCNB2

Entrez Gene IDs

9133 (Human); 12442 (Mouse); 363088 (Rat)

Gene Symbol