Human/Cynomolgus Monkey B7-H6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10162
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Cynomolgus Monkey
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1002910
Product Specifications
Immunogen
Human embryonic kidney cell, HEK293-derived Cynomolgus Monkey B7‑H6
Asp25-Ser262
Asp25-Ser262
Specificity
Detects human and cynomolgus monkey B7‑H6 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Cynomolgus Monkey B7-H6 Antibody
Detection of Cynomolgus Monkey B7‑H6 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or transfected with cynomologus monkey B7-H6. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7-H6 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of B7-H6 in HEK293 Human Cell Line Transfected with Human B7-H6 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with Human B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Detection of B7-H6 in HEK293 Human Cell Line Transfected with Cynomologus Monkey B7-H6 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with Cynomolgus Monkey B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Cynomolgus Monkey B7-H6 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Cynomologus Monkey or Human B7-H6 and eGFP
Sample: HEK293 Human Cell Line Transfected with Cynomologus Monkey or Human B7-H6 and eGFP
Western Blot
2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with with cynomologus monkey B7-H6
Sample: HEK293T human embryonic kidney cell line transfected with with cynomologus monkey B7-H6
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H6
References
- Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
- Li, Y. et al. (2011) J. Exp. Med. 208:703.
- Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:6223.
- Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
- Byrd, A. et al. (2007) PLoS ONE 2:e1339.
- Matta, J. et al. (2013) Blood 122:394.
- Schlecker, E. et al. (2014) Cancer Res. 74:3429.
- Zhou, Y. et al. (2015) Int. J. Clin. Exp. Pathol. 8:9428.
Long Name
B7 Homolog 6
Alternate Names
B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1
Gene Symbol
NCR3LG1
Additional B7-H6 Products
Product Documents for Human/Cynomolgus Monkey B7-H6 Antibody
Product Specific Notices for Human/Cynomolgus Monkey B7-H6 Antibody
For research use only
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