| Specificity: |
Detects a synthetic peptide specific for Cytokeratin 17 around amino acid 120 in Direct ELISA.
Applications for Human Cytokeratin 17 Antibody
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Recommended Concentration
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Sample |
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Western Blot |
2 µg/mL |
MDA-MB-468 human breast cancer cell line and A431 human epithelial carcinoma cell line |
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Simple Western |
100 µg/mL |
MDA-MB-468 human breast cancer cell line |
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Immunohistochemistry |
3-25 µg/mL |
Immersion fixed paraffin-embedded sections of human prostate and of human squamous cell carcinoma |
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Immunocytochemistry |
3-25 µg/mL |
fixed MDA-MB-468 human breast cancer cell line (Positive) and absent in Ramos human Burkitt's lymphoma cell line (Negative) |
Scientific Data Examples for Human Cytokeratin 17 Antibody
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Western Blot
Detection of Human Cytokeratin 17 by Western Blot.
Western Blot shows lysates of MDA‑MB‑468 human breast cancer cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Cytokeratin 17 Monoclonal Antibody (Catalog # MAB11571) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cytokeratin 17 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Immunohistochemistry
Detection of Cytokeratin 17 in Human Prostate.
Cytokeratin 17 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cytokeratin 17 Monoclonal Antibody (Catalog # MAB11571) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunohistochemistry
Detection of Cytokeratin 17 in Human Squamous Cell Carcinoma.
Cytokeratin 17 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Cytokeratin 17 Monoclonal Antibody (Catalog # MAB11571) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry
Detection of Cytokeratin 17 in MDA-MB-468 cells (Positive) and Ramos cells (Negative).
Cytokeratin 17 was detected in fixed MDA‑MB‑468 human breast cancer cell line (Positive) and absent in Ramos human Burkitt's lymphoma cell line (Negative) using Mouse Anti-Human Cytokeratin 17 Monoclonal Antibody (Catalog # MAB11571) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Simple Western
Detection of Human Cytokeratin 17 by Simple WesternTM.
Simple Western shows lysates of MDA‑MB‑468 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for Cytokeratin 17 at approximately 53 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human Cytokeratin 17 Monoclonal Antibody (Catalog # MAB11571). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human Cytokeratin 17 Antibody
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