Human red blood cells were stained with Mouse Anti-Human DARC PE-conjugated Monoclonal Antibody (Catalog # FAB4139P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human DARC PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human red blood cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: DARC

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Long Name

Duffy Antigen, Receptor For Chemokines

Alternate Names

ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy

Entrez Gene IDs

2532 (Human); 13349 (Mouse)

Gene Symbol

ACKR1

UniProt

Q16570

Additional DARC Products

Product Documents for Human DARC PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DARC PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human DARC PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4139P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all DARC Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DARC PE-conjugated Antibody

Detection of DARC in Human Red Blood Cells by Flow Cytometry.