Human Decorin Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB11475R
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 1072709
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585
Gly17-Lys359
Accession # P07585
Specificity
Detects human Decorin in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Decorin Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Titration recommended for optimal concentration
with starting range of 0.1-1 µg/1 million cells. Sample used for this
experiment was HEK293
cell line transfected with human Decorin.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium
Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Decorin
Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 - 12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.
References
- Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
- Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
- Danielson, K. et al. (1993) Genomics 15:146.
Alternate Names
DCN, DSPG2, PG-II, PGS2, SLRR1B
Gene Symbol
DCN
UniProt
Additional Decorin Products
Product Specific Notices for Human Decorin Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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