Human Dishevelled-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3316

R&D Systems, part of Bio-Techne
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AF3316
AF3316-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Dishevelled-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Dishevelled-1
Arg150-Leu215
Accession # O14640

Specificity

Detects human Dishevelled-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) DVL‑2 and rhDVL‑3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Dishevelled-1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Western Blot

0.1 µg/mL
Sample: Recombinant Human Dishevelled-1

Reviewed Applications

Read 3 reviews rated 3.7 using AF3316 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dishevelled-1

DVL-1 (Dishevelled-1) is a cytoplasmic phosphoprotein that is one of the three human orthologs of the Drosophila dishevelled segment polarity protein. Human DVL-1 is expressed in multiple tissues where it participates in Wnt signal transduction. DVL-1 is a modular protein containing DIX, PDZ and DEP linker regions that connect Wnt/Frizzled engagement to proteins involved in the inactivation of beta-catenin destruction in the Wnt canonical pathway. Human DVL-1 shows 93% aa identity with mouse and rat DVL-1 over the sequence used for immunization. Three shorter isoforms have been described, but it is unclear whether these are actually expressed.

Alternate Names

Dishevelled1, Dsh, DVL1

Entrez Gene IDs

1855 (Human); 13542 (Mouse); 83721 (Rat)

Gene Symbol

DVL1

UniProt

O14640

Additional Dishevelled-1 Products

Product Documents for Human Dishevelled-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Dishevelled-1 Antibody

For research use only

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