Human DLX5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6710
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DLX5
Asn216-Tyr289
Accession # P56178
Asn216-Tyr289
Accession # P56178
Specificity
Detects human DLX5 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DLX5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human DLX5 Antibody
DLX5 in Human Mesenchymal Stem Cells.
DLX5 was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Sheep Anti-Human DLX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6710) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human DLX5 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into osteocytes
Sample: Immersion fixed human mesenchymal stem cells differentiated into osteocytes
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DLX5
DLX5 (distal-less homeobox 5) is a 33-35 kDa member of the distal-less homeobox family of transcription factors. It is expressed in embryonic mesenchyme where it promotes cartilage and bone formation, and in nervous tissue, where it promotes interneuron development. Human DLX5 is 289 amino acids (aa) in length. It contains one DNA-binding homeobox domain (aa 137-196), and two phosphorylation sites at Ser34 and Ser217 that, when utilized, increases transcriptional activity. DLX5 likely forms homodimers, and is known to heterodimerize with MSX-2. Over aa 216-289, human DLX5 shares 99% aa identity with mouse DLX5.
Long Name
Distal-less Homeobox 5
Alternate Names
distal-less homeo box 5, distal-less homeobox 5, homeobox protein DLX-5
Gene Symbol
DLX5
UniProt
Additional DLX5 Products
Product Documents for Human DLX5 Antibody
Product Specific Notices for Human DLX5 Antibody
For research use only
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