Human DNER Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3646
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DNER
Arg29-His637
Accession # Q8NFT8
Arg29-His637
Accession # Q8NFT8
Specificity
Detects human DNER in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DNER Biotinylated Antibody
Detection of Human DNER by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human DNER Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line
Sample: Immersion fixed A172 human glioblastoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)
Western Blot
0.2 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DNER
Long Name
Delta/Notch-like EGF Repeat-containing Transmembrane
Alternate Names
BET, delta and Notch-like epidermal growth factor-related receptor, delta/notch-like EGF repeat containing, delta-notch-like EGF repeat-containing transmembrane, H_NH0150O02.1, UNQ26, WUGSC:H_NH0150O02.1
Gene Symbol
DNER
UniProt
Additional DNER Products
Product Documents for Human DNER Biotinylated Antibody
Product Specific Notices for Human DNER Biotinylated Antibody
For research use only
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