Human Dopamine D2 R/DRD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9266
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (Common Squirrel Monkey)
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 960710
Product Specifications
Immunogen
Human DRD2 synthetic peptide
Accession # P14416
Accession # P14416
Specificity
Detects human Dopamine D2 R/DRD2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Dopamine D2 R/DRD2 Antibody
Dopamine D2 R/DRD2 in Human Brain.
Dopamine D2 R/DRD2 was detected in immersion fixed paraffin-embedded sections of human brain (striatum) using Mouse Anti-Human Dopamine D2 R/DRD2 Monoclonal Antibody (Catalog # MAB9266) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Dopamine D2 R/DRD2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (striatum)
Sample: Immersion fixed paraffin-embedded sections of human brain (striatum)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine D2 R/DRD2
Dopamine receptor D2 (DRD2) is localized to human striatum, motor cortex and neocortex. It is a highly conserved seven transmembrane receptor member of the G-protein coupled receptor 1 family, with three known isoforms. In the striatum, DRD2 suppresses voluntary activity in the striatopallidal pathway, and polymorphisms in this gene are associated with alcohol addiction, smoking behavior, schizophrenia, food addiction and post-traumatic stress disorder as well as myoclonus dystonia. DRD2 has been shown to heterodimerize with DDR4 and 5-HT2A receptors, and traffics between the plasma membrane and intracellular pools and this localization can be modulated by several drugs. DRD2 is localized to human striatum, motor cortex and neocortex.
Long Name
Dopamine D2 Receptor
Alternate Names
D2DR, D2R, Dopamine D2R, DRD2
Gene Symbol
DRD2
UniProt
Additional Dopamine D2 R/DRD2 Products
Product Documents for Human Dopamine D2 R/DRD2 Antibody
Product Specific Notices for Human Dopamine D2 R/DRD2 Antibody
For research use only
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