Human Dopamine D5R/DRD5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8286
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 889040
Product Summary for Human Dopamine D5R/DRD5 Antibody
Immunogen
NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Accession # P21918
Accession # P21918
Specificity
Detects HEK293 cells transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Dopamine D5R/DRD5 Antibody
Dopamine D5 R/DRD5 in Human Brain.
Dopamine D5 R/DRD5 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB8286) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Dopamine D5R/DRD5 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Sample: Immersion fixed paraffin-embedded sections of human brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine D5R/DRD5
References
- Sunahra, R. et al. (1991) Nature. 350:614.
- Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
- Squassina, A. et al. (2008) Neurosci Lett. Feb 13.
- Golimbet, V. et al. (2008) Bull Exp Biol Med. Jan.
- Wei, J. et al. (2012) Addict Behav. 37:622.
- Thompson, D. et al. (2011) Traffic. 12:644.
Long Name
Dopamine D5 Receptor
Alternate Names
DBDR, Dopamine D5 R, DRD1B, DRD1L2, DRD5
Gene Symbol
DRD5
UniProt
Additional Dopamine D5R/DRD5 Products
Product Documents for Human Dopamine D5R/DRD5 Antibody
Product Specific Notices for Human Dopamine D5R/DRD5 Antibody
For research use only
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