Human DOT1L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6546
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 653613
Product Specifications
Immunogen
E. coli-derived recombinant human DOT1L
Gly2-Lys416
Accession # Q8TEK3
Gly2-Lys416
Accession # Q8TEK3
Specificity
Detects human DOT1L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PCDH-8, rhNME-3, or rhWISP-3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human DOT1L Antibody
DOT1L in Human Lung.
DOT1L was detected in immersion fixed paraffin-embedded sections of human lung using Human DOT1L Monoclonal Antibody (Catalog # MAB6546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human DOT1L Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung
Sample: Immersion fixed paraffin-embedded sections of human lung
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with recombinant human DOT1L, see our available Western blot detection antibodies
Sample: Cell lysates spiked with recombinant human DOT1L, see our available Western blot detection antibodies
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB6546 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DOT1L
Long Name
Disruptor of Telomeric Silencing 1-like
Alternate Names
H3-K79-HMTase, KMT4
Entrez Gene IDs
84444 (Human)
Gene Symbol
DOT1L
UniProt
Additional DOT1L Products
Product Documents for Human DOT1L Antibody
Product Specific Notices for Human DOT1L Antibody
For research use only
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