Human DPPII/QPP/DPP7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3438
Key Product Details
Species Reactivity
Validated:
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 398011
Product Summary for Human DPPII/QPP/DPP7 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPPII/QPP/DPP7
Gly22-Leu492
Accession # ABM85563
Gly22-Leu492
Accession # ABM85563
Specificity
Detects human DPPII/QPP/DPP7 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human DPPII/QPP/DPP7 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Recombinant Human DPPII/QPP/DPP7 (0.1 µg/mL, Catalog # 3438-SE) cleavage of the fluorogenic peptide substrate KP-AMC (10 µM). The Neutralization Dose (ND50) is typically 0.12 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPII/QPP/DPP7
Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The human DPP7 precursor consists of a signal peptide (aa 1‑21) and a mature chain (aa 22‑492). The purified rhDPP7 is active against Lys‑Pro‑AMC and Lys‑Ala‑AMC. Its activity against Lys‑Pro‑AMC is approximately 10‑fold of that against Lys‑Ala‑AMC under otherwise identical conditions.
References
- Underwood, R. et al. (1999) J. Biol. Chem. 274:34053.
- Maes, M.B. et al. (2005) Biochem. J. 386:315.
- Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
- Lankas, G.R. et al. (2005) Diabetes 54:2988.
Long Name
Dipeptidyl-peptidase II
Alternate Names
DPP7, QPP
Gene Symbol
DPP7
UniProt
Additional DPPII/QPP/DPP7 Products
Product Documents for Human DPPII/QPP/DPP7 Antibody
Product Specific Notices for Human DPPII/QPP/DPP7 Antibody
For research use only
Loading...
Loading...
Loading...