Human DSPG3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3110

R&D Systems, part of Bio-Techne
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MAB3110
MAB3110-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1053309

View all DSPG3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DSPG3
Ala20-Val322
Accession # Q99645

Specificity

Detects human DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DSPG3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human DSPG3 Antibody

DSPG3 antibody in Human HUVEC by Immunocytochemistry (ICC).

DSPG3 in Human HUVEC.

DSPG3 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human DSPG3 Monoclonal Antibody (Catalog # MAB3110) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DSPG3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human DSPG3 (Catalog # 3110-DS)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSPG3

Human DSPG3 (also PG-Lb and epiphycan) is a 95‑100 kDa class III subfamily member of the SLRP family of molecules. It is synthesized as a 322 aa precursor that contains a 19 aa signal sequence and a 303 aa mature region. The mature molecule contains two intrachain disulfide bonds that flank six leucine-rich repeats lying between aa 135-301. There is a potential for both O- and N-linked glycosylation. DSPG3 is found principally in cartilage. Mature human DSPG3 is 85%, 96%, 95% and 84% aa identical to rat, dog, cow and mouse DSPG3, respectively.

Long Name

Dermatan Sulfate Proteoglycan 3

Alternate Names

Epiphycan, EPYC, PG-Lb, SLRR3B

Entrez Gene IDs

1833 (Human); 13516 (Mouse)

Gene Symbol

EPYC

UniProt

Q99645

Additional DSPG3 Products

Product Documents for Human DSPG3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DSPG3 Antibody

For research use only

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