Human EBI3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB64561
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 607215
Product Summary for Human EBI3 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EBI3
Arg21-Lys229
Accession # Q14213
Arg21-Lys229
Accession # Q14213
Specificity
Detects human EBI3 in ELISAs for IL-39 detection.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human EBI3 Antibody
Human IL-39 ELISA Standard Curve.
Recombinant Human IL-39 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-23 p19 Monoclonal Antibody (MAB17161) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (Catalog # MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human EBI3 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑23 p19 Monoclonal Antibody (Catalog # MAB17161).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EBI3
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.
Long Name
Epstein-Barr virus induced gene 3
Alternate Names
EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3
Gene Symbol
EBI3
UniProt
Additional EBI3 Products
Product Documents for Human EBI3 Antibody
Product Specific Notices for Human EBI3 Antibody
For research use only
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