Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A) and either (A) Mouse Anti-Human EMR3 Monoclonal Antibody (Catalog # MAB8496) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

EMR3 in Human Lung Cancer Tissue.

EMR3 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human EMR3 Monoclonal Antibody (Catalog # MAB8496) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human EMR3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3

Alternate Names

egf-like module containing, mucin-like, hormone receptor-like 3, EGF-like module receptor 3, EGF-like module-containing mucin-like hormone receptor-like 3, egf-like module-containing mucin-like receptor 3

Entrez Gene IDs

84658 (Human)

Gene Symbol

ADGRE3

UniProt

Q9BY15

Additional EMR3 Products

Product Documents for Human EMR3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human EMR3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human EMR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8496

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all EMR3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human EMR3 Antibody

Detection of EMR3 in Human Blood Monocytes by Flow Cytometry.