Human EN-RAGE/S100A12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10522
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 161212
Product Specifications
Immunogen
E.coli-derived recombinant human EN-RAGE/S100A12
Met1-Glu92
Accession # NP_005612
Met1-Glu92
Accession # NP_005612
Specificity
Detects human EN-RAGE/S100A12 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human EN-RAGE/S100A12 Antibody
Detection of Human EN‑RAGE/S100A12 by Western Blot.
Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 2 µg/mL of Human EN-RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10522) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human EN-RAGE/S100A12 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human granulocytes
Sample: Human granulocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EN-RAGE/S100A12
EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa secreted molecule that belongs to the S100 family of calcium-binding proteins. EN-RAGE contains two EF-hand domains and forms dimers and hexamers in solution. EN-RAGE is overexpressed at sites of inflammation and interacts with RAGE on endothelial cells, lymphocytes, and monocytes to promote inflammatory responses. Human EN-RAGE shares 66% and 70% amino acid sequence identity with bovine and porcine EN-RAGE, respectively; mouse and rat orthologs have not been identified.
Long Name
Extracellular Newly Identified RAGE-binding Protein
Alternate Names
CAAF1, CAAFI, CAGC, CAGCS100, Calgranulin C, ENRAGE, MRP6, S100A12
Entrez Gene IDs
6283 (Human)
Gene Symbol
S100A12
UniProt
Additional EN-RAGE/S100A12 Products
Product Documents for Human EN-RAGE/S100A12 Antibody
Product Specific Notices for Human EN-RAGE/S100A12 Antibody
For research use only
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