Human Endorepellin/Perlecan Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2364
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endorepellin/Perlecan
Arg3684-Ser4391 (Ser4003Thr)
Accession # P98160
Arg3684-Ser4391 (Ser4003Thr)
Accession # P98160
Specificity
Detects human Endorepellin/Perlecan in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Endorepellin/Perlecan Antibody
Endorepellin/Perlecan in Human Skin.
Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/ Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Endorepellin/Perlecan Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Western Blot
0.1 µg/mL
Sample: Recombinant Human Endorepellin/Perlecan
Sample: Recombinant Human Endorepellin/Perlecan
Reviewed Applications
Read 5 reviews rated 4 using AF2364 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endorepellin/Perlecan
Long Name
Heparan Sulfate Proteoglycan
Alternate Names
HSPG2, Perlecan
Gene Symbol
HSPG2
UniProt
Additional Endorepellin/Perlecan Products
Product Documents for Human Endorepellin/Perlecan Antibody
Product Specific Notices for Human Endorepellin/Perlecan Antibody
For research use only
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