Human Endostatin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1098

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
BAF1098
Print Quote
Bulk Order
Conjugate
Catalog #
Unconjugated
AF1098
View Product
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Endostatin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Endostatin
His1572-Ser1753
Accession # P39060

Specificity

Detects human Endostatin in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse Endostatin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Endostatin Biotinylated Antibody

Endostatin antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

Endostatin in Human Kidney Cancer Tissue.

Endostatin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Endostatin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1098) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Endostatin Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer

Western Blot

0.1 µg/mL
Sample: Recombinant Human Endostatin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endostatin

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

Alternate Names

COL18A1

Entrez Gene IDs

80781 (Human); 12822 (Mouse)

Gene Symbol

COL18A1

UniProt

P39060

Additional Endostatin Products

Product Documents for Human Endostatin Biotinylated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Endostatin Biotinylated Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.