Human Endothelin-1 C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB34401
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2E1
Product Summary for Human Endothelin-1 C-Terminus Antibody
Immunogen
Human Endothelin-1
Specificity
Detects human Endothelin-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Endothelin-1 C-Terminus Antibody
Human Endothelin‑1 ELISA Standard Curve.
Recombinant Human Endothelin-1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Endothelin-1 C-Terminus Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB3440).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Endothelin Pan Specific DuoSet ELISA Kit (Catalog # DY1160) for convenient development of a sandwich ELISA or the Endothelin-1 Quantikine ELISA Kit (Catalog # DET100) for a complete optimized ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endothelin-1
Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).
Alternate Names
ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME
Gene Symbol
EDN1
Additional Endothelin-1 Products
Product Documents for Human Endothelin-1 C-Terminus Antibody
Product Specific Notices for Human Endothelin-1 C-Terminus Antibody
For research use only
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