Human Endothelin-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5579
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3B6
Product Summary for Human Endothelin-3 Antibody
Immunogen
Human Endothelin-3 synthetic peptide
Accession # P14138
Accession # P14138
Specificity
Detects human Endothelin‑3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Endothelin-3 Antibody
Endothelin‑3 in LNCaP Human Cell Line.
Endothelin-3 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Endothelin-3 Monoclonal Antibody (Catalog # MAB5579) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Endothelin-3 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line
Sample: Immersion fixed LNCaP human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endothelin-3
Endothelin-3 is further cleaved by Kell blood group antigen endopeptidases to generate the 21 amino acid (aa) bioactive mature peptide. It interacts primarily with the 7TM receptor EDNRB to induce a variety of effects including bronchoconstriction, arteriolar vasoconstriction, gall bladder contraction, insulin and norepinephrine release, and the maintenance and development of melanocytes and neural crest progenitors. Mature human Endothelin-3 shares 100% aa sequence identity with mouse and rat Endothelin-3.
Alternate Names
EDN3, Endothelin3
Gene Symbol
EDN3
UniProt
Additional Endothelin-3 Products
Product Documents for Human Endothelin-3 Antibody
Product Specific Notices for Human Endothelin-3 Antibody
For research use only
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