Human ENPP-7/Alk-SMase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4924
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Ala22-Ser439
Accession # Q6UWV6
Ala22-Ser439
Accession # Q6UWV6
Specificity
Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ENPP-7 is observed, and less than 5% cross-reactivity with recombinant human (rh) ENPP-2 and rhENPP-5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human ENPP-7/Alk-SMase Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ENPP-7/Alk-SMase (Catalog # 4924-EN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ENPP-7/Alk-SMase (Catalog # 4924-EN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human ENPP-7/Alk-SMase (Catalog # 4924-EN)
Sample: Recombinant Human ENPP-7/Alk-SMase (Catalog # 4924-EN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ENPP-7/Alk-SMase
ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (alk-SMase) is expressed in the intestines and in human bile (1). It shares 30%‑36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP-7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies also show a decrease in ENPP-7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP-7 in human colon cancer (4, 5).
References
- Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
- Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
- Wu, J. et al. (2006) Biochem J. 394:299.
- Hertervig, E. et al. (1996) Cancer. 79:448.
- Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 7
Alternate Names
Alk-SMase, ENPP7, NPP7
Gene Symbol
ENPP7
UniProt
Additional ENPP-7/Alk-SMase Products
Product Documents for Human ENPP-7/Alk-SMase Antibody
Product Specific Notices for Human ENPP-7/Alk-SMase Antibody
For research use only
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